Congratulations to Beth and the team for publishing a strong, thoughtful, and evidence-based reply to an earlier comment in Molecular Ecology.
The take-home: there are a lot of challenges and opportunities when it comes to using dietary DNA metabarcoding strategies to advance a variety of important research agendas; Beth is leading the way when it comes to clear-thinking about how we conduct our studies and how we can strengthen the evidence we use to support our conclusions.
The exemplary professional, Beth exposes persistent and potentially problematic misconceptions in the field in a way that is clear, constructive, and self-reflective. Whether or not our decisions about how to code dietary DNA metabarcoding data have a qualitative influence on our ecological interpretations is a question that we should all be asking. Beth and the team provide invaluable insight into when we might need to pay particular attention to this issue, and offer a blueprint to help us address the issue in a more structured way by sharing computer code that we wrote to conduct simulation studies and sensitivity analyses with real data.
Littleford-Colquhoun BL, Sackett VI, Tulloss CV, Kartzinel TR. 2022. Evidence-based strategies to navigate the complexity of dietary DNA: a reply. Molecular Ecology doi: 10.1111/mec.16712. [PDF]