Conservation & Molecular Ecology @Brown
  • Home
  • People
  • Publications
  • Research
  • Conservation
  • News
  • Join
  • Contact

Bioinformatics Workshop Archive

Initial processing of Illumina data for dietary DNA metabarcoding

1/20/2023

0 Comments

 
By Bethan Littleford-Colquhoun

A huge debt of gratitude to Beth for organizing a google drive folder that shares our strategy for initial processing of dietary DNA metabarcoding data. This directory provides template documents and code that we use to download paired-end read data from our dietary metabarcoding workflows, assemble forward and reverse reads, perform initial quality controls, and evaluate sequencing success. There is also a tutorial for how to upload sample data to SRA for archiving, which we have started to do routinely. Some details are specific to our dietary analysis sequencing workflow and/or the specific sequencing service that we use, plus we have particular sample naming schemes that we use, but it should be pretty straightforward to adapt for a variety of similar goals.
0 Comments

    Author

    Computational resources kindly contributed and explained by members of our community.

    Archives

    January 2023
    December 2022
    November 2022
    January 2022
    June 2021
    January 2021
    March 2019
    February 2019
    January 2019

    Categories

    All
    Dada2
    Data
    Data Management
    DNA Barcodes
    High-performance Computing
    Lab Protocols
    Metabarcoding
    Microbiome
    Oscar
    Phylogenetics
    Pipelines
    Reference Libraries
    R Tutorials
    Software

    RSS Feed


Picture
Copyright 2021 © Tyler Kartzinel

  • Home
  • People
  • Publications
  • Research
  • Conservation
  • News
  • Join
  • Contact