Rolling Out a Curated Set of Software and Data RepositoriesOver the past couple of years, a lot of things have changed. Some changes have improved how we share the software and data that we generate in the lab, necessitated in part by the growing popularity of our work. We recognized a substantial benefit to enhancing our commitment to providing easy access and open-source principles and therefore made it easier to find and access our most in-demand repositories across a variety of platforms that include GitHub, Zenodo, NCBI, BOLD, etc. You can now easily find links with descriptions from our main Software & Data landing page. Check it out!
0 Comments
Map-Making with Hillshade in ROur most recent map-making tutorial focuses on unlocking the potential of rasters. You can open Map-making for ecologists, tutorial 3: Rasters: combining them, adding hillshade, and suppressing legends here or access the Map-making in R GitHub repository to download the data and code directly.
Make a Map of Your Study Sites with an Inset in ROur most recent map-making tutorial shares a useful workflow for tailoring map designs to different types of studies. You can open Map-making for ecologists, tutorial 2: Inset maps here or access the Map-making in R GitHub repository to download the data and code directly.
Map-Making in R for Ecologists
Bioinformatic Strategies for Abundance FilteringOver the years, our lab has contributed a number of essential reviews about how DNA sequence data can be accurately converted into dietary information. The science is clear: inappropriate assumptions about how to 'clean up' sequence data using bioinformatics can do more harm than good by warping our diet profiles and generating misleading assumptions. Nevertheless, we have to make some such assumptions to generate datasets that are useful and informative. How should we think about striking a balance between these competing imperatives?
Led by Dr. Bethan Littleford-Colquhoun, one of the more important reviews we've produced on this topic was published in Molecular Ecology: The Precautionary Principle. This review, and a follow-up reply describing Evidence-based Strategies to Navigate Complexity, tackle the challenge of identifying appropriate abundance-filtering strategies in DNA metabarcoding pipelines. This post provides an essential summary of what we found... Building a Plant DNA Barcode Library: Fieldwork EditionThis post provides resources and advice from the Kartzinel Lab to help you plan and build a plant DNA reference library to use in dietary metabarcoding projects, which evidence has shown can markedly improve results. We recently did the math and found that our team has produced some of the most extensive and widely used DNA barcode libraries in the world, with experience from Kenyan savannas, Yellowstone National Park, arid scrublands of Chile, and many other places around the world.
Simple Phylogenetics Workflow for DNA BarcodesOne great application of DNA barcodes is the ability to generate accurate and relevant phylogenetic trees for ecological and evolutionary analyses. There are lots of ways to do this, but not all of them may be necessary or relevant to your end goals. What do you need to know before you get started? This post provides a simple road map that you can follow to decide whether and how to construct a phylogeny using DNA barcode data for your research.
Plant DNA Barcode Library for Mpala Research CentreFor more than 13 years, we have built and maintained a plant DNA Barcode library for the flora of Mpala Research Centre in Kenya. Many versions have been released, the most substantial of which was associated withe 2019 publication of the Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna in Molecular Ecology. This post is intended to help you track significant developments in this long-term collaborative effort and find the most current version to use in your analyses.
High-Performance Computing on the OSCAR SupercomputerBrown University's high performance computing cluster, called Oscar, is a keystone resource for research in the Kartzinel Lab. The resource and information about it are regularly updated; users may find the links provided in this post to be especially helpful as they are getting started.
|

RSS Feed