Featured Software from the Kartzinel Lab: Geographic Coverage of DNA Barcodes. The inaugural code repository to be highlighted in our Featured Software section of the Software & Data page presents the Quarto Code Book published in association with our Molecular Ecology Review Paper, "Global Availability of Plant DNA Barcodes as Genomic Resources to Support Basic and Policy-Relevant Biodiversity Research" can be easily modified to evaluate the geographic coverage of other data sets. Although the featured code emphasizes geographic coverage from our work in Yellowstone National Park...

Over the past couple of years, a lot of things have changed. Some of those changes have brought improvements to the way we share the software and data that we generate in the lab, necessitated in part by the growing popularity of our work to a point that we recognized a substantial benefit to enhancing our commitment to easy access and open-source principles. 

To make it easier to find and access our most in-demand repositories -- often spread across a variety of platforms including GitHub, Zenodo, NCBI, BOLD, etc. -- we have created a main landing page on the website where we have curated a mix of both recent and timeless contributions to the field. Check it out!

You might also want to check out our lab's main GitHub repository, where we are increasingly making our projects publicly available so you can benefit from any updates we've made since posting a version of record for the code with a prior publication (e.g., a static record with a Zenodo DOI). 

I will periodically post updates to this blog in order to highlight and summarize important updates to help ensure the community is aware, but we will no longer be using these pages to share data or code directly as we have in the past.

By Hannah Hoff

We have been doing a lot of plant DNA barcoding to build a library for the plants of the Greater Yellowstone Ecosystem. In the process, we have done some refinement to our lab's DNA barcoding protocols to increase clarity, efficiency, and reproducibility. Updated links to these protocols are available on the lab's wiki under the "Plant Barcoding" section for: trnL, rbcL, matK, and trnH-psbA markers.

Collaborator Nick Harvey has kindly provided a formatted version of our current Mpala Plant DNA Barcode Reference Library that is suitable for taxonomic assignments using the R package dada2. You can download the fasta file of reference library v.2.0 (corresponding to Gill et al. 2019) formatted for dada2 here.

​Thank you, Nick, for making this time-saving resource available to share!

We are often asked to provide advice or assistance building plant DNA reference libraries for use in dietary metabarcoding projects. To begin centralizing info on our methods and sharing some important lessons-learned from experience, I have created a section on the lab's wiki for building plant barcode libraries. I will treat the google docs that you can link to from there as living documents. All of the details provided are nested within two main goals. The first goal is to collect plant voucher specimens and plant DNA barcode samples that match in ways that can be clearly documented  through their respective metadata sheets. This is critical for the long-term value of the data. The second goal is to ensure work done by field biologists and molecular biologists are mutually informative -- the best reference libraries are developed through the meaningful engagement of expert botanists who are knowledgeable in a local flora and the researchers who will be analyzing the laboratory data. 

We love to archive relevant vouchers in the Brown University Herbarium. Please keep in mind that the herbarium is staffed by expert botanists. Properly collected specimens can be mounted, archived, and digitized by professional staff -- this greatly reduces the cost and complexity of fieldwork.