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Bioinformatics Workshop

We have curated our most popular Software & Data repositories so you can find them easily

Our Lab's GitHub site also provides useful info and resources related to current projects

Preparing dietary DNA data files for publication

10/9/2025

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Preparing Dietary DNA Data for Manuscript Files

Working with dietary DNA metabarcoding data? Unsure how to concisely summarize your workflow for publication? Tired of all the effort required to format your data tables for archiving in Dryad, supplementary materials, or other archives? The lab has posted new code to our GitHub repository that will help you solve all of these problems.

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Our standard DNA metabarcoding pipeline updated and posted for 2025

7/17/2025

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Our Standard DNA Metabarcoding Pipeline

Fans of the lab will be very excited to see this much-anticipated release of our standard dietary DNA metabarcoding pipeline, with a walk-through easily accessible on the centralized "Software & Data" section of our webpage. Until now, people would have to access code repositories associated with each of our publications or contact us directly to model their analysis after our well-established workflow. That led to multiple versions of the pipeline in circulation, since we are constantly improving it and published versions quickly ended up out of date. We have tried to solve that problem by...
Diet metabarcoding pipeline overview based on tutorial and workflow from the Kartzinel Lab and CCV at Brown University

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Bioinformatic strategies for abundance filtering

1/20/2022

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Bioinformatic Strategies for Abundance Filtering

Over the years, our lab has contributed a number of essential reviews about how DNA sequence data can be accurately converted into dietary information. The science is clear: inappropriate assumptions about how to 'clean up' sequence data using bioinformatics can do more harm than good by warping our diet profiles and generating misleading assumptions. Nevertheless, we have to make some such assumptions to generate datasets that are useful and informative. How should we think about striking a balance between these competing imperatives?

Led by Dr. Bethan Littleford-Colquhoun, one of the more important reviews we've produced on this topic was published in Molecular Ecology: The Precautionary Principle. This review, and a follow-up reply describing Evidence-based Strategies to Navigate Complexity, tackle the challenge of identifying appropriate abundance-filtering strategies in DNA metabarcoding pipelines. This post provides an essential summary of what we found... 

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New release of trnL-P6 reference data for Mpala Research Centre

2/27/2019

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Plant DNA Barcode Library for Mpala Research Centre

For more than 13 years, we have built and maintained a plant DNA Barcode library for the flora of Mpala Research Centre in Kenya. Many versions have been released, the most substantial of which was associated withe 2019 publication of the Plant DNA-barcode library and community phylogeny for a semi-arid East African savanna in Molecular Ecology. This post is intended to help you track significant developments in this long-term collaborative effort and find the most current version to use in your analyses.

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Dr. Tyler Kartzinel
Department of Ecology, Evolution, and Organismal Biology
Institute at Brown for Environment and Society
Brown University
​Address: 85 Waterman Street, Providence, Rhode Island 02912 USA
Office: 246(B)
​Lab (pre-PCR): 244
​Lab (post-PCR): 230
​Phone: 1-401-863-5851
tyler_kartzinel[at]brown.edu
Disclaimer: views expressed on this site are those of the author. They should not be interpreted as opinions or policies held by his employer, collaborators, or lab members. Mention of trade names or commercial products does not constitute endorsement.

Copyright 2017-2026 © Tyler Kartzinel
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  • Home
  • Research
    • DNA metabarcoding
    • Conservation Genetics
    • Yellowstone
    • Fray Jorge
    • Savanna Ecology
    • Molecular Parasitology
  • Resources
    • Publications
    • Software & Data
    • Protocols
    • News
    • Bioinformatics Workshop
  • Impact
    • Conservation
    • Annual Reports
    • Donate
  • Work with us
    • People
    • Join
    • Contract & Collaborate >
      • DNA metabarcoding contracts
      • DNA barcoding
      • Training
  • Contact